Developmental Biology Laboratory

Immunolocalization of molecular markers in cells, tissues, and embryos.

Keywords: animals, developmental biology, EvoDevo (Evolutionary Developmental Biology), germline, mitochondria, electrophoresis, Western Blot, immunofluorescence, fluorescent probes, confocal microscopy.

 

Teaching and research on biological samples. Specifically, they are incubated with antibodies for immunocytochemistry and immunofluorescence studies. Samples are also treated with dyes (nuclear or organelle-specific) to identify cell types and study cellular function. The specificity of the antibodies used is tested by Western Blot after acrylamide gel electrophoresis (SDS-PAGE) of tissue homogenates. 

 

Equipment available in the laboratory

The Developmental Biology Laboratory is equipped with a hood, oven, hotplate, magnetic stirrer, vortex mixer, swing plate, mechanical roller, pH meter, technical balance, pipettors, centrifuge, and a fluorescence microscope. There is also access to SDS-PAGE and Western Blot equipment. 

 

Teaching, training and research activities

In the Development Biology laboratory, undergraduate and Master's degree students carry out curricular and thesis internships. Furthermore, the laboratory contributes to the development of research projects of Italian and foreign PhD students and post-doctoral fellows.

 

Contacts

Album

On the left: Monodimensional SDS-PAGE of three different concentrations of the same protein extract stained with Coomassie blue. On the right: Western blot of the same samples using anti-Vasa antibody.

Immunofluorescence on germinal bed of Podarcis sicula: germ cells at different stages of differentiation are identified using anti-Vasa antibody and To-Pro-3 nuclear dye.

General view of the Developmental Biology Lab

General view of the Developmental Biology Lab

General view of the Developmental Biology Lab

General view of the Developmental Biology Lab